Journal: BMC gastroenterology

Article Title: The impact of probiotic supplementation on gastric motility and nutrient absorption in elderly patients with Gastrointestinal disorders.

doi: 10.1186/s12876-025-03740-2

Figure Lengend Snippet: Fig. 3 Comparison of intestinal permeability markers levels between two groups (after treatment 7d). (A) Occludin; (B) Zonulin; (C) LBP. LBP: Lipopolysac charide Binding Protein. *: P < 0.05; **: P < 0.01.Paired t-tests were used to compare pre- vs. post-treatment values within each group; independent t-tests were used for between-group comparisons at day 7

Article Snippet: The specific ELISA kits employed included: the human zonulin kit (EKC 36091, Biomatik USA, LLC; Wilmington, DE, USA), the human occludin kit (NBP2-80305, Novus Biologicals, LLC; Centennial, CO, USA), and the human LBP kit (DY870-05, R&D Systems, Inc.; Minneapolis, MN, USA).

Techniques: Comparison, Permeability, Binding Assay

Journal: Advanced healthcare materials

Article Title: Engineering of Uniform Epidermal Layers via Sacrificial Gelatin Bioink-Assisted 3D Extrusion Bioprinting of Skin.

doi: 10.1002/adhm.202301015

Figure Lengend Snippet: Figure 4. Comparison of sacrificial gelatin-assisted extrusion bioprinting with the manual seeding method. A) Schematic representing the experimental groups: 1) Sacrificial gelatin bioink-assisted extrusion bioprinting (Bioprinted); 2) Manual seeding of HEK-laden culture medium (Manual). The central and peripheral areas of the respective groups were observed to observe HEK adhesion. Confocal images stained with actin filament (F-actin) and E- cadherin at the B) central and C) peripheral regions. Scale bar: 25 μm. D) Quantitative result of keratinocyte-occupied area within the rectangular framework. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results using E) early markers for keratinocyte differentiation and F) E-cadherin mRNA expression indicating adherens junctions between keratinocytes. G) Quantification of protein expression levels for human occludin, ZO-1, and claudin-1, representative tight junction proteins, using enzyme-linked immunosorbent assay (ELISA). *p < 0.01 when compared to that of the manual group (n = 5).

Article Snippet: Commercially available ELISA kits were employed to quantify the protein levels of human occludin (EEL-H1073; Elabscience, USA), human tight junction protein ZO-1 (E-ELH1516; Elabscience), and human claudin-1 (MBS725675; MyBioSource, USA), according to the manufacturer’s instructions.

Techniques: Comparison, Staining, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Gut Microbes

Article Title: The probiotic Bacillus subtilis BS50 decreases gastrointestinal symptoms in healthy adults: a randomized, double-blind, placebo-controlled trial in healthy adults

doi: 10.1080/19490976.2022.2122668

Figure Lengend Snippet: Plasma lipids, intestinal permeability markers, and inflammation markers at baseline (day –1) and end of the supplementation period (day 42).

Article Snippet: Plasma zonulin (catalog no. EKC 36091, Biomatik USA, LLC; Wilmington, DE, USA), occludin (catalog no. NBP2-80305, Novus Biologicals, LLC; Centennial, CO, USA), and LBP (catalog no. DY870-05, R&D Systems, Inc.; Minneapolis, MN, USA) were analyzed in duplicate by ELISA by the Institute for Food Safety and Health (Illinois Institute of Technology; Chicago, IL, USA) according to manufacturer’s instructions.

Techniques: Clinical Proteomics, Permeability

Journal: Nutrients

Article Title: Implications of Indirect Biomarkers of Intestinal Permeability in the Stools of Newborns and Infants with Perinatal Risk Factors for Intestinal Colonization Disorders and Infant Feeding Patterns

doi: 10.3390/nu14112224

Figure Lengend Snippet: Values of fecal lipopolysaccharide (LPS), occludin and zonulin in the examined children (nonparametric Mann–Whitney test).

Article Snippet: The determinations of the studied parameters were performed via the immunoenzymatic method using ready-made reagent kits: Lipopolysaccharide (LPS)—LPS ELISA Kit (EIAab), Human Occludin (OCLN) ELISA Kit (EIAab) and Human Zonulin ELISA Kit (Cat. No E3704Hu) from Bioassay Technology Laboratories (BT LAB) (sensitivity: 0.13 ng/mL standard curve range: 0.3–90 ng/mL).

Techniques:

Journal: Nutrients

Article Title: Implications of Indirect Biomarkers of Intestinal Permeability in the Stools of Newborns and Infants with Perinatal Risk Factors for Intestinal Colonization Disorders and Infant Feeding Patterns

doi: 10.3390/nu14112224

Figure Lengend Snippet: Analysis of dependent variables.

Article Snippet: The determinations of the studied parameters were performed via the immunoenzymatic method using ready-made reagent kits: Lipopolysaccharide (LPS)—LPS ELISA Kit (EIAab), Human Occludin (OCLN) ELISA Kit (EIAab) and Human Zonulin ELISA Kit (Cat. No E3704Hu) from Bioassay Technology Laboratories (BT LAB) (sensitivity: 0.13 ng/mL standard curve range: 0.3–90 ng/mL).

Techniques: